Figure 6: The observed growth arrests correlate with accumulation of precursor proteins. | Scientific Reports

Figure 6: The observed growth arrests correlate with accumulation of precursor proteins.

From: Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei

Figure 6

Immunoblots showing the steady-state levels of CoxIV and mtHsp70 in the uncomplemented and complemented ATOM14 3′UTR RNAi cell lines. Cytosolic EF1a serves as a loading control. Time of induction in days is indicated at the top. The position of precursor (p) and mature forms (m) are indicated. The following cell lines were used: (A) ATOM14 3′UTR RNAi cell line, (B) ATOM14 3′UTR RNAi cell line ectopically expressing wildtype ATOM14, (C) ATOM14 3′UTR RNAi cell line ectopically expressing an ATOM14 variant lacking the IMS domain and (D) ATOM14 3′UTR RNAi cell line ectopically expressing an ATOM14 variant lacking the cytsosolic domain.

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