Figure 8: The membrane domain of ATOM14 mediates assembly of the ATOM complex.

Digitonin-extracted crude mitochondrial fractions of the indicated uninduced (−Tet) and induced (+Tet) transgenic T. brucei cell lines were separated by BN-PAGE. The resulting immunoblots were probed using antisera against ATOM40 (top panels) and ATOM46 (bottom panels), respectively. The following cell lines were used: ATOM14 3′UTR RNAi cell line, ATOM14 3′UTR RNAi cell line ectopically expressing wildtype ATOM14 (ATOM14), ATOM14 3′UTR RNAi cell line ectopically expressing an ATOM14 variant lacking the IMS domain (ATOM14-ΔIMS) and ATOM14 3′UTR RNAi cell line ectopically expressing an ATOM14 variant lacking the cytsosolic domain (ΔCYT-ATOM14). Vertical lines on the right side of the panels indicate the position of the high molecular weight ATOM complexes. Asterisks on the right side of the panels indicate the position of the ATOM core complex.