Figure 2: Testing of TOP-PCR on 166 bp fragment and PhiX DNA sample. | Scientific Reports

Figure 2: Testing of TOP-PCR on 166 bp fragment and PhiX DNA sample.

From: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

Figure 2

(a) Size distribution of the original and TOP-PCR amplified 166-bp TBC1D1 DNA fragment profiled by Fragment Analyzer. (Black, original 166-bp fragment sample, 1 ng loaded; blue, after 20-cycle TOP PCR amplification of 1 ng of the original, 1 ng loaded to the FA device). The peak of the original TBC1D1 fragment should be 166 bp in size, but is shown as a 173 bp peak due to the imprecision of the device. (b) Size distribution of the original and TOP-PCR amplified PhiX DNA fragments profiled by FA. (Black, original PhiX control DNA, 2.8 ng loaded; blue, after 20-cycle TOP-PCR amplification of 1 ng of the original, 4.6 ng loaded). Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

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