Figure 5: Test of TOP-PCR reproducibility and cfDNA consistency. | Scientific Reports

Figure 5: Test of TOP-PCR reproducibility and cfDNA consistency.

From: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

Figure 5

Two blood samples were separately collected from a healthy male (YFH) on June 30, 2015 and October 28, 2015. Plasmas were prepared right after the blood collections and stored at −80 °C. Samples of cfDNA were extracted right before TOP-PCR reactions conducted on October 28, 2015. Blue, plasma stock prepared on June 30, 2015; red and black, plasma stock prepared on October 1, 2015. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

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