Figure 6: Comparison of TOP-PCR with Illumina’s PCR method using low amount of DNA as the input. | Scientific Reports

Figure 6: Comparison of TOP-PCR with Illumina’s PCR method using low amount of DNA as the input.

From: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

Figure 6

(a) One micro-liter of original ovarian cancer plasma cfDNA sample with unknown concentration. (b) Same DNA sample but after 50 cycles of amplification by TOP-PCR. (c) Same DNA sample but after 50 cycles of amplification using Illumina’s protocol. Size markers: 35 bp and 6000 bp peaks. Size of two HAs added to each DNA fragment: ~22 bp.

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