Table 1 Sequence analysis of TOP-PCR and Illumina PCR products amplified from equal amount of ovarian cancer plasma cfDNA with undetectable quantity.

From: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids

PCR Method

#/insert within pZBack flanking seq.

#Seq.

Insert within adaptors

Illumina

2 had no reports (from Sanger sequencing)

  

8 with loop adaptor seq.

0

H. sapiens

2

E. coli

6

unknown

5 empty (with pZBack flanking seq. only)

  

5 unknown sequences

  

TOP-PCR

13 with HA seq.

8

H. sapiens seq.

3

E. coli seq.

1

Aeromonas veronii (a bacterium) seq.

1

unknown seq.

6 empty (with pZBack flanking seq. only)

  

1 unknown sequence

  
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