Figure 1: Position and organization of the subventricular cellular cluster. | Scientific Reports

Figure 1: Position and organization of the subventricular cellular cluster.

From: Identification of novel cellular clusters define a specialized area in the cerebellar periventricular zone

Figure 1

(A) and (C), toluidine blue stain of 1-mm and 40-μm (respectively) coronal cerebellar slices; an arrow indicates the location of the SVCC in the CbPVZ. (B) a black line indicates the location of the SVCC in a sagittal plane, and below (C), it is shown in the coronal plane. (D). White arrowheads point to cells positive for GAD-65 in the SVCC. (E) Cells expressing the neuronal NeuN marker are distributed in the SVCC (white arrowheads); some of the cells are in vicinity with the terminal end feet of Bergmann glia (yellow arrowheads in E and white arrowheads in F). (G) Calretinin-positive cells are distributed throughout the SVCC (white arrowheads); the insert shows an amplification. (H) The SVCC does not include calbindin-positive cells (arrow), in contrast to Purkinje neurons (arrowheads). (I) Vimentin label was found in a population of ependymal glial cells (red arrowhead) but absent in the SVCC. (J) SVCC includes cells positive for nestin (orange arrowheads), and is delimited dorsally by Bergmann glia (yellow arrowheads) and ventrally by ependymal cells (green arrowheads). Cb: cerebellum; GL: granular layer; PCL: Purkinje cellular layer; ML: molecular layer; CbPVZ: cerebellar periventricular zone; IV: fourth ventricle; PI: propidium iodide; CALRET: calretinin; GFAP: glial fibrillar acidic protein; Calb: calbindin; GAD-65: glutamate decarboxylase isoform 65.

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