Figure 2: Evidence of neurons in and around the SVCC.
(B) Cells labeled by MBP are distributed through the SVCC (white arrowheads), and the VMC is disclosed by the expression of GFP (arrow in C). (D) Transmission electron microscopy showed the presence of transversal and longitudinal axons (black and yellow arrowheads), the presence of multiciliated cells indicated the presence of ependymal cells (green arrowheads). A sagittal representation of the site of AdCherry administration (black line in panel A); yellow and green lines indicate the coronal planes represented in A1 and A2. (E) mCherry randomly labeled cells in the SVCC (white arrowheads); several cells present long processes (yellow arrowheads) that extend laterally. The VMC is indicated with an arrow (E,F), and it includes GFAP+(in green) and GFAP- cells, some cells labeled with mCherry (orange arrowhead in G) and several cells labeled with both fluorescent proteins. In (F) Individual cell labeling was obtained with mCherry (white arrowhead) and identified one cell extending a long projection laterally (yellow arrowhead); in addition, GFAP + cells, morphologically similar to astrocytes, project several processes to the VMC (orange arrowheads) whereas others contact the terminal feet of Bergmann glia (purple arrowheads). In (G) an orthogonal plane of 2F is presented. GFAP: glial fibrillar acidic protein; AdCherry: adenoviral vector carrying the fluorescent protein Cherry gene; CbPVZ: cerebellar periventricullar zone.
