Figure 3: Distribution of ventromedial cord on the roof of the fourth ventricle.

The distribution of the VMC on lobe I (I) is indicated with an arrow in (A,B,C and D) In A GFAP+cells are aligned ventromedially; additionally, several individual glial cells with large somas were found lateral to the VMC (white arrowheads). (B) Cells positive for nestin are distributed in the VMC (white arrowheads), and in (C) white arrowheads point to some nestin+cells in the VMC (labeled in red), and yellow arrowheads indicate nestin+cells lateral to the VMC. (D) Phaloidine-rhodamine showed the surface of the roof of the fourth ventricle in the GFAP-GFP transgenic mouse. The VMC is indicated by white arrowheads; two capillaries run along this structure (yellow arrowheads in D and white arrow in E), and they are contacted by long glial processes extending from the VMC (arrowheads in E). Some glial cells with radial morphology extend longitudinally along the interface between the choroid plexus (ChP) and lobe I (gray arrowheads in D). (F) Pinwheel-like structures are present on the surface of the ventricle (red arrowheads in D and F). PI: propidium iodide; NES: nestin; GFAP: glial fibrillar acidic protein; I: lobe I; ChP: choroid plexus.