Figure 7: Development of the SVCC and VMC. | Scientific Reports

Figure 7: Development of the SVCC and VMC.

From: Identification of novel cellular clusters define a specialized area in the cerebellar periventricular zone

Figure 7

The VMC is observed from embryonic (E) day 15 (orange arrowheads in A) in a 2-mm sagittal section of a clarified GFAP-GFP cerebellum (see also supplementary videos). From P0 to P5 the SVCC and VMC expand, whereas from P10 the VMC is ventromedially limited to the ependymal glial cell layer (blue arrowheads in B-G stacks of 40 μm and optical slices from CLARITY B’–G’). From P5 to P30 the SVCC was found to be delimitated by the end feet of the Bergmann glia (yellow arrowheads in 40-μm stacks [C-G] and optical slices from CLARITY [C’–G’]) of transgenic GFAP-GFP mice (red nuclei labeled with propidium iodide). An example of clarified adult brain is presented in the bottom in A. IGL: Internal granular layer; PCL: Purkinje cellular layer, ML: Molecular layer, EGL: external granular layer, GL: granular layer; PVZ: Periventricular zone, ChP: Choroid plexus, P: postnatal stage. Barr: 50 μm.

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