Figure 2: Expression and sub-cellular localization of BORIS in colorectal cancer cell lines.
From: Brother of Regulator of Imprinted Sites (BORIS) suppresses apoptosis in colorectal cancer
(a) BORIS expression levels in colorectal cell lines were determined by western blot assay in the left panel, which were shown on cropped blots. Full-length blots and whole protein on nitrocellulose membrane were included in Supplementary Fig. S1a. The graph in the right panel represents the percentage of the signal in each fraction measured by densitometric analysis of the western blot. (b) BORIS expression levels in colorectal cancer cell lines were determined by using quantitative real-time PCR. (c) Localization of endogenous BORIS in HCT116 and Caco-2 cells. The right panels show the proportion of the signal distributed in the cytoplasm and in the nucleus.
