Figure 5: BORIS and cytoplasm-localized BORIS-ZFdel suppressed apoptosis and BORIS silencing induced ROS formation.
From: Brother of Regulator of Imprinted Sites (BORIS) suppresses apoptosis in colorectal cancer
(a) Caspase 3/7 activity induced by BORIS silencing was reduced by transfection with plasmids expressing BORIS or BORIS-ZFdel. The empty vector was used as an overexpression control. The Y axis indicates the difference in caspase 3/7 activity between BORIS siRNA- and negative control siRNA-transfected cells. (b) Transfection of pBORIS and pBORIS-ZFdel suppressed the release of cytochrome c induced by BORIS silencing. Cytochrome c was immunostained by using a mouse monoclonal cytochrome c antibody and FITC-conjugated secondary antibody. (c) Caspase 3/7 activity induced by H2O2 treatment was suppressed by either BORIS or BORIS-ZFdel overexpression. The Y axis indicates the difference in caspase 3/7 activity between H2O2 and H2O treated cells. (d) ROS production induced by siBORIS was suppressed by overexpression of BORIS, but not BORIS-ZFdel. The Y axis indicates induction of ROS by siBORIS treatment. Statistical differences between the control and treatments were evaluated by two-tailed Student’s t-test. *p < 0.05; **p < 0.01.
