Figure 6: Sensitivity of colorectal cancer cells to 5-FU is affected by BORIS expression.
From: Brother of Regulator of Imprinted Sites (BORIS) suppresses apoptosis in colorectal cancer
(a) Caco-2 cells were treated with BORIS siRNA together with 5-FU. The viability of the treated cells was evaluated. Negative control siRNA and DMSO-treated cells were used as controls. (b) The cell viability of BORIS-overexpressing Caco-2 cells treated with 5-FU was compared with that of controls. (c) Overexpression of pBORIS-ZFdel did not counteract the inhibitory effect of 5-FU or H2O2 on the proliferation of HCT116 cells. The statistical differences between the samples are evaluated by two-tailed Student’s t-test and presented in Supplementary Fig. S9. *p < 0.05; **p < 0.01; ***p < 0.001. The transfection efficiency of the siRNA and the plasmids is shown in Supplementary Fig. S10.
