Figure 3: PGC-1α induces the secretion of proteins from muscle cells to activate macrophages.
From: Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP
(A,B) mRNA expression levels of chemokines identified in the CM of C2C12 myotubes overexpressing PGC-1α by mass spectrometry were validated in C2C12 myotubes overexpressing PGC-1α (A) and MCKα mice (B). (C,D) Other identified factors from the CM potentially involved in the muscle-macrophage cross-talk were validated by RT-PCR. mRNA expression levels of LCN2, Pcolce and BNP were determined in C2C12 myotubes adenovirally overexpressing PGC-1α or GFP (C) and in WT and MCKα mice (D). Values represent the mean of at least 3 independent experiments or at least 6 animals +SEM. *P < 0.05; **P < 0.01; ***P < 0.001; PGC-1α versus GFP, MCKα versus WT.
