Figure 2: FP7 antagonizes LPS-induced human monocyte stimulation and DC maturation. | Scientific Reports

Figure 2: FP7 antagonizes LPS-induced human monocyte stimulation and DC maturation.

From: TLR4 antagonist FP7 inhibits LPS-induced cytokine production and glycolytic reprogramming in dendritic cells, and protects mice from lethal influenza infection

Figure 2

(a) Monocytes were isolated from peripheral human blood, stimulated for 24 h with increasing amounts of LPS or the same volume of PBS as control, in the presence of 10 μM FP7 or solvent (Solv). Data represents mean cytokine secretion in monocyte supernatants from 4 independent experiments. (b–d) Dendritic cells (DCs) were differentiated from human peripheral blood monocytes for 6 days. DCs were stimulated for 24 h with increasing amounts of LPS or the same volume of PBS as control, in the presence of 10 μM FP7 or solvent. Surface expression of DC maturation markers was monitored by flow cytometry and means ± SEM of fluorescence intensity are shown (b). Cytokine secretion was assayed in DC supernatants by Cytometric Bead Array (CBA). Data represents mean cytokine secretion from 6 independent experiments.

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