Figure 4: FP7 selectively antagonizes TLR4. | Scientific Reports

Figure 4: FP7 selectively antagonizes TLR4.

From: TLR4 antagonist FP7 inhibits LPS-induced cytokine production and glycolytic reprogramming in dendritic cells, and protects mice from lethal influenza infection

Figure 4

(a–c) HEK293/hTLR4-MD2-CD14, HEK293/hTLR1-TLR2, HEK293/hTLR2-TLR6 cells were treated 24 h with ultrapure LPS, peptidoglycan (PGN), or Pam3CSK4 (Pam3). Supernatants were collected and IL-8 secreted in response to TLR stimulation was assayed. (d) DCs were stimulated for 24 h with 10 ng/ml ultrapure LPS or 10 μg/ml PGN, PAM3, or pIC in the absence or presence of 10 μM FP7. Control cells (Ctl) received PBS instead of TLR ligand. Cytokine secretion was assayed in DC supernatants means ± SEM from 3 independent experiments are shown.

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