Table 1 Gp41-MinTT-containing immunogens used in this study.

From: Proteoliposomal formulations of an HIV-1 gp41-based miniprotein elicit a lipid-dependent immunodominant response overlapping the 2F5 binding motif

FORMULATION

LIPID COMPOSITION (molar ratio)

PROTEIN

DOSE/INOCULUM

Recombinant protein

 

gp41-MinTT

20 μg

Simple proteoliposomes

POPC (100)

gp41-MinTT

2 μg

POPC:GM3 (95:5)

gp41-MinTT

2 μg

POPC:PS (95:5)

gp41-MinTT

2 μg

POPC:GM3:PS (90:5:5)

gp41-MinTT

2 μg

Complex proteoliposomes

POPC:SM:CHOL (30:25:45)

gp41-MinTT

2 μg

POPC:SM:CHOL:GM3 (30:20:45:5)

gp41-MinTT

2 μg

POPC:SM:CHOL:PS (25:25:45:5)

gp41-MinTT

n.d.*

POPC:SM:CHOL:GM3:PS (25:20:45:5:5)

gp41-MinTT

2 μg

Complex proteoliposomes + MPLA

POPC:SM:CHOL:MPLA (29:25:45:1)

gp41-MinTT

2 μg

POPC:SM:CHOL:GM3:MPLA (29:20:45:5:1)

gp41-MinTT

2 μg

POPC:SM:CHOL:PS:MPLA (24:25:45:5:1)

gp41-MinTT

2 μg

POPC:SM:CHOL:GM3:PS:MPLA (24:20:45:5:5:1)

gp41-MinTT

2 μg

  1. Immunogens were categorized in recombinant protein (gp41-MinTT), simple (POPC), complex (POPC CHOL SM) and complex + MPLA (POPC CHOL SM MPLA) proteoliposomes. GM3, PS or both were added to each category. Percentages are referred to lipid components. Lipid mole ratios were used. POPC: 1-Palmitoyl-2-oleoylphosphatidylcholine; CHOL, cholesterol; SM, sphingomyelin; GM3, monosialodihexosylganglioside; PS, phosphatidylserine; MPLA, monophosphoryl lipid A.
  2. *n.d.: not determined.
Back to article page