Figure 4: M3 AChR mediates endogenous ACh-induced cell proliferation and phosphorylation of ERK1/2 and AKT. | Scientific Reports

Figure 4: M3 AChR mediates endogenous ACh-induced cell proliferation and phosphorylation of ERK1/2 and AKT.

From: Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation

Figure 4

After cells were treated with trihexyphenidyl (M1R antagonist), AFDX-116 (M2R/M4R antagonist), and 4-DAMP (M3R antagonists) for 5 days at indicated concentrations, CCK-8 assay was used to detect cell growth in MKN45 (a) and BGC823 cells (b). (c) Western blot showed the expression changes of p-ERK1/2 and p-AKT in MKN45 and BGC823 cells under 0.01 μM to 10 μM of 4-DAMP treatment for 4 h. (*P < 0.05; **P < 0.01).

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