Figure 4: Differences in the global genomic DNA methylation in Lizixiang and ND98 under salt stress.

(A) Heat maps showing the expression of the genes related to DNA methylation or demethylation. (B) Differential expression of DNA demethylation-related genes validated using qRT-PCR. IbROS1, c144343.graph_c0; IbDML3, c148603.graph_c0. (C) MSAP profiling analysis showing different patterns of locus-specific DNA methylation in Lizixiang and ND98. The gel shows the results for one primer combination; twenty EcoRI + HpaII/MspI primer combinations were used in total. H, HpaII; M, MspI. Differentially methylated sites between Lizixiang and ND98 are indicated with arrows. L0, Lizixiang 0 h; L12, Lizixiang 12 h; L48, Lizixiang; N0, ND98 0 h; N12, ND98 12 h; and N48, ND98 48 h.