Figure 6: The key animo acid residue affected the ability of JcARF19 binding with JcIAA9.
From: Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas
(a) Predicated secondary structure of JcIAA9, JcARF19 and other related proteins. (b) GST pull-down assay. Recombinant bait protein GST-JcIAA9 COOH-terminal (residues 229–368 shows JcIAA9-CT containing protein-protein interaction domain III and domain IV of Aux/IAA protein family) strongly binds to prey protein JcARF19 COOH-terminal (residues 1005–1276), but weakly binds to prey protein JiARF19-CT (residues 1008–1279). (c) BiFC assay. Very strong fluorescence signal was only found in the combination of C-terminal of JcIAA9 and JcARF19 (nYFP-JcIAA9+JcARF19-cYFP), but not in the combination of C-terminal of JcIAA9 and JiARF19 (nYFP-JcIAA9+JiARF19-cYFP). Bar: 50 μm.
