Figure 4: PLIN2 accumulation enhances the ER stress of β cells. | Scientific Reports

Figure 4: PLIN2 accumulation enhances the ER stress of β cells.

From: PLIN2 is a Key Regulator of the Unfolded Protein Response and Endoplasmic Reticulum Stress Resolution in Pancreatic β Cells

Figure 4

(A,B) MIN6 cells were transduced with control or Plin2-retrovirus. PLIN2 overexpression was examined by (A) quantitative RT-PCR (n = 4) and (B) immunoblotting and quantification. GAPDH was used as a loading control. (C) Quantitative RT-PCR. Control and Plin2-overexpression MIN6 cells treated with vehicle or 5 μg/ml tunicamycin (TM) for 6 hours (n = 4–6). (D,E) Total RNA and protein were collected from control wild-type (WT) β cells and Akita β cells (n = 5–6). PLIN2 expression was examined by (D) quantitative RT-PCR and (E) immunoblotting and quantification. GAPDH was used as a loading control. (F,G) Control and Plin2-knockdown Akita β cells were treated with vehicle or 5 μg/ml TM for 6 hours (n = 4–6). (F) Immunoblotting and quantification. GAPDH was used as loading control. (G) Quantitative RT-PCR. All immunoblotting quantification was normalized to vehicle/control. *p < 0.05.

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