Figure 7: PLIN2 modulates autophagic flux in pancreatic β cells.

(A) Immunoblotting and quantification. Control and Plin2-knockdown MIN6 cells were treated with vehicle or 5 μg/ml tunicamycin (TM) for 6 hours. GAPDH was used as loading control. (B) Control and Plin2-knockdown MIN6 cells were transfected with LC3-GFP construct and subquently treated with vehicle or 0.1 mM chloroquine (CQ) for 6 hours. Nuclei were stained with DAPI (blue). The number of LC3-GFP punctae (green dots) was quantified. Total of 50 GFP-positive cells were calculated each group. (C) Immunoblotting and quantification. Wild-type control and Akita β cells were treated with vehicle or 5 μg/ml TM for 6 hours. GAPDH was used as loading control. (D) Immunoblotting and quantification. Cells were treated with vehicle or 0.1 mM CQ for 6 hours. GAPDH was used as loading control. All immunoblotting quantification was normalized to scramble-shRNA vehicle. *p < 0.05. Scale bar = 5 μm.