Figure 4: CCT binding to soluble α-synuclein A53T oligomers. | Scientific Reports

Figure 4: CCT binding to soluble α-synuclein A53T oligomers.

From: The chaperonin CCT inhibits assembly of α-synuclein amyloid fibrils by a specific, conformation-dependent interaction

Figure 4

(a) Left, SEC analyses of CCT, α-syn A53T oligomers and the mixture of both. Coloured lines at bottom mark the different peaks. Right, SDS-PAGE of the samples eluted in the left panel, after silver staining, colour-coded as in the elution profile. Red and green arrows indicate bands corresponding to CCT subunits and to α-syn A53T monomers, respectively. (b) XL-MS of the crosslinked CCT-α-syn A53T soluble oligomer complex. Top view of CCT atomic structure (pdb ID code 4B2T) showing residues identified as crosslinked to α-syn A53T (red).

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