Figure 2: ChR2 activation induces inward current and membrane depolarization of smooth muscle cells.
From: Optogenetic Modulation of Urinary Bladder Contraction for Lower Urinary Tract Dysfunction
(a) Phase contrast and YFP imaging of ChR2-expressing SMC. Scale bar, 20 µm. (b) Representative traces of inward currents evoked by blue light at 0.0014, 0.014, 0.14, 1.41, 1.94 (mW/mm2). Relationship between inward currents versus light intensities (n = 5). (c) Representative traces of membrane potential changes evoked by blue light at 0.0014, 0.014, 0.14, 1.41, 1.94 (mW/mm2). Relationship between peak membrane potential changes versus light intensities (n = 5). (d) I-V curve of the photocurrents evoked by ChR2 (n = 5). The reversal potential was around + 20 mV. All error bars indicate SE.
