Figure 8: Comparison of bladder function between ChR2(H134R) and SSFO(C128S/D156A) gene transfections.
From: Optogenetic Modulation of Urinary Bladder Contraction for Lower Urinary Tract Dysfunction
Bladders of C57BL/6J mice were injected either with ad-CMV-hCHR2(H134R)-EYFP or ad-CMV-hCHR2(C128S/D156A). (a) Representative traces of pressure changes in H134R-bladder (black) and SSFO-bladder (red) in response to 1 s continuous illumination of blue light. At the onset of blue light illumination, H134R-bladder contraction quickly rose to a peak pressure, then decayed to the baseline level (black), whereas SSFO-bladder produced an initial peak pressure followed by a reduced, but elevated plateau pressure until yellow illumination deactivated the SSFO (red). Blue bar indicates the blue light illumination for activation (473 nm, 63 mW and 1 s continuous illumination) and yellow bar, the yellow light illumination for deactivation of SSFO (589 nm, 189 mW and 2 s continuous illumination). (b) The average peak and plateau pressure changes after 1 s illumination (n = 5). All error bars indicate SE.
