Figure 3: HO-1 inhibits adipogenesis in primary adipose precursor cells isolated from mice fed a brief HFD. | Scientific Reports

Figure 3: HO-1 inhibits adipogenesis in primary adipose precursor cells isolated from mice fed a brief HFD.

From: HO-1 inhibits preadipocyte proliferation and differentiation at the onset of obesity via ROS dependent activation of Akt2

Figure 3

All visceral and subcutaneous APs were isolated from mice on a SD or HFD for 3 days via Sca-1 bead pull down (see ‘Experimental Procedures’). (A,B) Oil-Red O staining of scAP or viAP cell cultures derived from Hmox1fl/fl and Hmox1fl/flPdgfraCre mice 7 days after the induction of adipocyte differentiation (n = 3). (C) Flow cytometry for viAP cell cultures derived from Hmox1fl/fl and Hmox1fl/flPdgfraCre mice Bodipy lipid-stained 4 days after the induction of adipocyte differentiation, measured as mean fluorescence intensities (n = 3). Results are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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