Figure 4: Icaritin modulates the expression profile of genes in relation to stem cell signaling. | Scientific Reports

Figure 4: Icaritin modulates the expression profile of genes in relation to stem cell signaling.

From: Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ERα

Figure 4

mESCs were exposed to 10 nM Icaritin for 24 h. Total RNA was extracted, reverse transcribed and amplified in PCR arrays as indicated in Materials and Methods. The experiment was performed in triplicates. (a) Hierarchical clustering analysis on the PCR array data. (b) A summary of top ten up-regulated and down-regulated genes in mESCs following Icaritin treatment compared with the controls. (c) Protein levels of p130 and CDX2 after Icaritin treatment were examined by Western blot analysis. β-actin was used as a loading control. Shown are cropped from the original blots.

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