Figure 6: p130 modulates the expression of Cyclin E and pluripotency transcription factors. | Scientific Reports

Figure 6: p130 modulates the expression of Cyclin E and pluripotency transcription factors.

From: Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ERα

Figure 6

mESCs were transfected with control (Coni) or p130 (p130i) siRNA followed by further analysis. (a) Protein levels of Cyclin E and CDK2 in response to p130 gene knockdown was detected by Western blot analysis. Protein (b) and mRNA (c) expression of OCT4, NANOG, SOX2, KLF4 in mESCs following p130 knockdown was examined by Western blot and quantitative real-time PCR respectively. Western blot shown are cropped from the original blots. (d) Representative images of ALP+ colonies at day 5 following siRNA mediated p130 knockdown in mESCs. (e) Quantitation of ALP+ colonies at day 5 following p130 knockdown. (f) BrdU incorporation assay for cell proliferation following siRNA mediated p130 knockdown in mESCs. Each treatment was performed in triplicate. Values were the mean ± SD (n = 3). *P < 0.05; **P < 0.01.

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