Table 1 Primer information for preparing DNA fragments with different length.
Primer symbol | Primer sequence (5′-3′) | Product length (bp) | Symbol of product with blunt ends | Symbol of product with 3′-A overhanging ends |
|---|---|---|---|---|
F | GAAGGTCGGAGTCAACGG | — | — | — |
R1 | AGGCTCGTAGACGCGGTT | 82 | T-1 | T-1A |
R2 | AGTAGGGACCTCCTGTTTCTG | 271 | T-2 | T-2A |
R3 | CGAAAGGAAAGAAAGCGTC | 326 | T-3 | T-3A |
R4 | GCTGCGCACTAGCATCCC | 408 | T-4 | T-4A |
R5 | AGGCTTTCCTAACGGCTG | 501 | T-5 | T-5A |
R6 | TGTCCTTTTCCAACTACCCA | 745 | T-6 | T-6A |
R7 | GAAATCAGGAGTGGGAGCA | 1073 | T-7 | T-7A |
R8 | GGAAGATGGTGATGGGATTT | 1943 | T-8 | T-8A |
