Figure 1: Representative pictures from phase contrast and immunofluorescence staining of neonatal rat ventricular cardiomyocytes after 24 h culture. | Scientific Reports

Figure 1: Representative pictures from phase contrast and immunofluorescence staining of neonatal rat ventricular cardiomyocytes after 24 h culture.

From: Modification of distinct ion channels differentially modulates Ca2+ dynamics in primary cultured rat ventricular cardiomyocytes

Figure 1

After 24 h, the cultured cardiomyocytes displayed characteristics of elongated and triangular shape (Phase Contrast). The immunocytochemistry demonstrated that the percentage of cardiomyocytes in our culture system was greater than 95% using a specific cardiomyocytes maker antibody (anti-cTnT) and Hoechst double staining. The experiments were repeated in three independent cultures with similar results.

Back to article page