Figure 3: Colocalization of CCNI2 and CDK5 in transiently transfected cells. | Scientific Reports

Figure 3: Colocalization of CCNI2 and CDK5 in transiently transfected cells.

From: Cyclin I-like (CCNI2) is a cyclin-dependent kinase 5 (CDK5) activator and is involved in cell cycle regulation

Figure 3

COS-7 cells were transfected with expression vectors that express human CCNI, CCNI2 and CDK5 with GFP or mCherry tag. (A) When expressed alone, mCherry-CDK5 localizes diffusely in the cell. (B) GFP-CCNI mainly localizes in the nuclei. (C) GFP-CCNI2 mainly localizes in the cytoplasm and the plasma membrane. (D) When expressed together with GFP-CCNI, mCherry-CDK5 moves into the nuclei and colocalizes with CCNI. (E) When expressed together with GFP-CCNI2, mCherry-CDK5 mainly localizes in the cytoplasm as well as on the plasma membrane. Nuclei were stained with DAPI. The fluorescent intensity was quantified using Image J. Scale bar: 20 μm.

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