Figure 1: Estradiol does not affect RelA translocation into the nucleus. | Scientific Reports

Figure 1: Estradiol does not affect RelA translocation into the nucleus.

From: Understanding the dynamics of Toll-like Receptor 5 response to flagellin and its regulation by estradiol

Figure 1

MCF7 cells were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin. (a) Time-lapse confocal images of a typical RelA-dsRedxp translocation after flagellin stimulation. (b) Time to nuclear RelA peak in MCF7 cells transiently transfected with RelA-dsRedxp. (c) Time course of RelA-dsRedxp normalized amplitude in transiently transfected responding MCF7 cells after flagellin stimulation. (d) Percentage of RelA-dsRedxp transiently transfected MCF7 cells responding to flagellin. (e) Amplitude and area of RelA-dsRedxp transiently transfected MCF7 cells after flagellin stimulation. (f) SEAP expression of MCF7 cells transiently transfected with pNifty2-SEAP incubated with 100 ng/ml of flagellin for 0, 15, 30, 45 min or 24 h. SEAP expression was evaluated 24 h post flagellin stimulation. NF-κB transcription activity was normalized against the control group stimulated for 24 h. Data are representative of 3 independent experiments with more than 20 cells per experiment (A–D) or at least five independent experiments (E). Error bars denote SEM (d,f) or SD (b,c,e). *P < 0.05.

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