Figure 2: Estradiol does not affect TLR5-mediated IκBα expression. | Scientific Reports

Figure 2: Estradiol does not affect TLR5-mediated IκBα expression.

From: Understanding the dynamics of Toll-like Receptor 5 response to flagellin and its regulation by estradiol

Figure 2

MCF7 cells were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin. (a) Western blot of IκBα and α Tubulin of MCF7 cells. (b) Real-time qPCR analysis in IκBα gene expression of MCF7 cells cultured in the presence of 10 nM E2 or not (control) and stimulated with 100 ng/ml of flagellin for 0, 0.5, 2 and 6 h before collection. Data are representative of at least 3 independent experiments. Error bars denote SEM.

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