Figure 2: The expression of fusogenic protein syncytin-1 in SCC-9 and its receptor ASCT-2 were increased by TNF-α. | Scientific Reports

Figure 2: The expression of fusogenic protein syncytin-1 in SCC-9 and its receptor ASCT-2 were increased by TNF-α.

From: Up-regulation of syncytin-1 contributes to TNF-α-enhanced fusion between OSCC and HUVECs partly via Wnt/β-catenin-dependent pathway

Figure 2

(a) Protein level of syncytin-1 was evaluated by western blot in SCC-9, MG-63 and Hela cell line. (b) Protein level of ASCT-2 was evaluated by western blot in HUVEC, SCC-9, MG-63 and MCF-7 cell line. (c,f) The difference of syncytin-1 and ASCT-2 expression in 6, 12, and 24 h between TNF-α stimulated group and control group. The expression of syncytin-1 in SCC-9 and ASCT-2 in HUVECs were continuous and dramatic highly expressed compared to the negative control group with 10 ng/ml TNF-α treatment. The expression of phosphorylation of β-catenin (P-β-catenin) decreased sharply in TNF-α group, while the amount of β-catenin increased evidently. (d) Positive expression of syncytin-1 in squamous cell carcinoma, 200× and 400×, respectively. The expression of syncytin-1 mainly localized in cellular membrane and/or cytoplasm, and the level of protein expression varied in different samples. (e) The level of protein expression was evaluated by score 0–7. 22 out of 28 samples (78.6%) showed moderate and high expression of syncytin-1 and scored 4–7; While others (21.4%) scored 0–3.

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