Figure 6: Effects of VHL on HSC activation, proliferation, and apoptosis.

(A) Immunocytochemistry for VHL (green fluorescence) and αSMA (red fluorescence) in HSCs. (B) HSC proliferation was measured (*p < 0.001). (C) HSC apoptosis was detected. (*p < 0.001). (D) Western blot analysis of p53, bcl-2 and cleaved caspase 3, and the gels have been run under the same experimental conditions.