Figure 5: A behavioral analysis of the PP-3copy-Aβ1-6-loop123 immunized APP/PS1 transgenic mice.

(a–i) Represent the Morris water maze test. For the cohorts treated before the onset of AD, the latencies are shown in (a) (p = 0.0122), total numbers of target platform crossings are shown in (b) (p = 0.0205), and the time spent by animals in the target quadrant are shown in (c). (a,b and c) Share the same set of tags. For the cohorts treated directly after the onset of AD, the latencies are shown in (d) (p = 0.0396), total numbers of target platform crossings are shown in (e) (p = 0.0409), and the time spent by animals in the target quadrant are shown in (f) (p = 0.0221). (d,e and f) share the same set of tags. For the cohorts treated long after the onset of AD, the latencies are shown in (g) (p = 0.0476), total numbers of target platform crossings are shown in (h), and the time spent by animals in the target quadrant are shown in (i). (g,h and i) Share the same set of tags. (j–n) Relate to the nest-building test. (j) Standard for evaluation. (k) Representative results of the nest-building test from the cohort immunized directly after the onset of AD. The quantitative analyze results from the cohort treated before the onset of AD (l) (p = 0.0441), directly after the onset of AD (m) (p = 0.0306), and long after the onset of AD (n) (p = 0.0417). All the results are expressed as mean values ± SEM. Number of animals per group is indicated in the bracket. ANOVA test was used to analyze the statistical significance of the data. *p < 0.05, NS = non-significant.