Figure 3: KRAS mutation analysis (direct sequencing) of codon 12 of exon 2.

(A) Hotspot of exon 2 shows KRAS wildtype, (B) KRAS mutation (p.G12D, arrow), or (C) low-level mutation (p.G12C, arrow, faint red signal), as confirmed by next generation sequencing (NGS) (case #190).