Figure 2: Structural analysis of mycobacterial SDR NAD binding sites.
From: Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
(A) Carveol dehydrogenase from M. paratuberculosis (MypaA.01326.b) bound to NAD (PDB ID 3PGX). The insertion loop which cover the NAD is shown in green and the loop often disordered in other SDR crystal structure is show in blue (left) and surface rendering showing the available solvent channel with hydrophobic regions shown in white, electropositive regions shown in blue and electronegative regions shown in red (right). (B) SDR from M. abscessus (MyabA.01326.f) bound to NAD (PDB ID 3S55). (C) FabG oxidoreductase from Staphylococcus aureus bound to NADPH (PDB ID 3SJ7)23. (D) FabG oxidoreductase from Bacillus sp. SG-1 bound to NAD (PDB ID 4NBU)24. This figure is intended to be representative examples of crystal structures of SDRs with the insertion loop determined here (panels A, B) with those bound to a more open NAD/NADP (panel C) and those bound to a more closed NAD/NADP (panel D); see Table 2 for calculated solvent accessibility of these and other structures.
