Figure 3: NMR spectroscopic analysis of mycobacterial SDR ligand binding.
From: Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Reference (blue) and saturation (red) STD NMR spectra overlaid for a M. avium SDR (SSGCID ID MyavA.01326.l) in the presence of (A) cofactor NADH (2.1% max STD signal), (B) potential product (S)-(+)-carvone (20.5% max STD NMR signal), (C) potential inhibitor tricyclazol (19.0% max STD NMR signal), and (D) redox partner DCIP (10.2% max STD NMR signal). Although NADH appears to be a non-binder under these conditions, the other compounds all show clear effects of binding.
