Table 6 STD-NMR binding analysis of compounds with M. avium carveol dehydrogenase MyavA.01326.l.
From: Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Compound | Description | Spike molecule | %STD (Day 0) | %STD cofactor spike (Day 0) | %STD cofactor spike (Day 7) |
|---|---|---|---|---|---|
NAD | Cofactor | N/A a | 3.6 | N/A | N/A |
NADH | Cofactor | N/A | 2.1 | N/A | N/A |
(−)-carveol | Substrate | NAD | 10.2 | 3.9 | 8.8 |
(R)-(−)-carvone | Product | NADH | 13.5 | 14.0 | 14.4 |
(S)-(+)-carvone | Product | NADH | 20.5 | 21.3 | 19.7 |
Tricyclazol | Inhibitor | NAD | 17.5 | 19.9 | 16.1 |
Tricyclazol | Inhibitor | NADH | 19.0 | 19.5 | 17.3 |
N,N-dimethyl-4-nitosoanaline | Redox partner | NADH | 7.5 | 8.3 b | 14.9 b |
2,6-dichloroindophenol (DCIP) | Redox partner | NAD | 11.4 | 11.5 | 13.8 |
2,6-dichloroindophenol (DCIP) | Redox partner | NADH | 10.2 | 3.0 b | 13.0 b |
