Figure 2
(a) Schematic illustration of a series of 100 basepair dsDNA structures featuring basepair-mismatched regions. Each dsDNA scaffold contains a nucleoprotein filament assembly site located adjacent to the mismatched region. The extension of the unpaired region between scaffolds is in the 3′ to 5′ direction (indicated by the green arrow) i.e. away from the patterning region. The nucleoprotein filament assembly site is indicated in red and the recognition site of the XapI enzyme is shown as a blue box. (b) Peak force tapping AFM image of a 100 bp DNA molecule with fully basepaired matching sequence (left) and a 100 bp DNA molecule containing a 20-basepair unpaired region located directly adjacent to the nucleoprotein filament assembly site (right). The height profiles for both DNA molecules, taken along the long axis of the molecule, are shown in Figure S2.
