Figure 5: Yield of RecA-mediated sequence-specific assembly onto dsDNA scaffolds containing 0, 2, 6, 8, 10, 12, 15 and 20 basepair mismatches, each centered 10 bp away from the patterning site. | Scientific Reports

Figure 5: Yield of RecA-mediated sequence-specific assembly onto dsDNA scaffolds containing 0, 2, 6, 8, 10, 12, 15 and 20 basepair mismatches, each centered 10 bp away from the patterning site.

From: Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks

Figure 5

The yield was obtained via an XapI enzyme digestion assay. The schematic diagrams of three of the different 100 bp dsDNA scaffolds used (containing 2, 10 and 20 bp mismatches, centered 10 bases from the nucleoprotein filament patterning region (indicated in red)) are shown in the inset.

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