Figure 1: MoDCs show higher rate of apoptosis than PDDCs. | Scientific Reports

Figure 1: MoDCs show higher rate of apoptosis than PDDCs.

From: Human IDO-competent, long-lived immunoregulatory dendritic cells induced by intracellular pathogen, and their fate in humanized mice

Figure 1

(A) Unpulsed MoDC and Pg-wt differentiated DC (WT-PDDC) were stained with propidium iodide staining solution using apoptosis detection kit (Annexin V staining kit, eBioscience). Cells were washed in RPMI supplemented with 10% FBS, and cytospin in order to perform immunofluorescence assay. Apoptotic MoDC and PDDC (arrows), with a characteristic condensed, fragmented, brighter nucleus than non-apoptotic DCs. Insets, view of PI staining of representative apoptotic cells. Scale bars: DCs, 35 μm; Monocytes (MN), 15 μm, (B) Unpulsed MoDC and Pg-wt differentiated DC (WT-PDDC) were stained with propidium iodide staining solution using apoptosis detection kit (Annexin V staining kit). Percentage of MN, MoDCs and PDDCs that present condensed or fragmented nuclei (PI) or the outer membrane (annexin+), (C) PDDCs generated from E. Coli LPS treatment or infection with Mfa-1+ strains (WT, DPG) displays a significantly lower level of surface Annexin V staining compared to starved monocytes and MoDC controls. This significant decrease is lost when cells are pretreated with HIV-1gp120 to block DC-SIGN prior to infection or PDDCs are generated with Mfa-1 deficient strains (MFI, MFB). These results are the mean of three independent experiments (n = 3).

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