Figure 2: Phosphorylated Foxo1 decreases with PDDC apoptosis rate. | Scientific Reports

Figure 2: Phosphorylated Foxo1 decreases with PDDC apoptosis rate.

From: Human IDO-competent, long-lived immunoregulatory dendritic cells induced by intracellular pathogen, and their fate in humanized mice

Figure 2

(A) FOXO1 expression was observed on protein level in PDDCs (WT & DPG) by carrying out Western blot using Anti-FOXO1 Mab. α-Tubulin, loading control (n = 2). The amount of total PDDC Foxo1 (B) and phosphorylated Foxo1 (C) were measured by whole cell ELISA and normalized to total cell numbers (n = 3) (B) PDDCs generated with E. coli LPS or either of the P. gingivalis strains have significantly higher levels of Foxo1. (C) PDDCs generated by the minor-fimbriae expressing WT and DPG-3 or the non-fimbriated MFB have significantly higher levels of phosphorylated Foxo1 (D) DCs were washed in RPMI and then incubated for 10 hrs in 10% FBS in RPMI. DCs were then lysed and Bim was detected by immunoblot. α-tubulin, loading control (n = 2).

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