Table 2 Monocyte-derived DCs (MoDCs) and Pathogen (P. gingivalis) differentiated dendritic cells (PDDCs) regulate expression of various markers relative to monocytes (Fold regulation shown in selected genes which are expressed on MoDC and PDDC).

From: Human IDO-competent, long-lived immunoregulatory dendritic cells induced by intracellular pathogen, and their fate in humanized mice

Gene markers

Fold change in gene expression in MoDC

Relative fold change in gene expression in pathogen (P. gingivalis) differentiated DC

WT-PDDC (Wild type)

DPG-PDDC (Minor Fimbriae)

MFI-PDDC (Major Fimbriae)

MFB-PDDC (Double mutant)

CD80

2

1

11.2

8.9

8.3

CD83

3.4

0.9

1

4.1

2.1

CD86*

8.15

0.3

2.8

0.2

0.2

CD40*

17.85

1.1

4.3

5.85

4.6

CD275

2.7

0.55

4.4

1.05

0.2

CD274

10.5

11

23

123

65

CD39

16.56

0.494

1.2

0.95

0.31

IL-1β*

0

4.65

41.9

16.55

8.7

IL-6*

0

16

48

312

231

IL-10*

2.15

35.6

32.1

65.35

62.05

IL-12β

1.3

34.85

5.5

34.4

93.7

IFNγ

0

1.9

0

14.6

41.71

TGFBR2

8.5

0.55

1.5

0.52

0.245

SOCS 1

10

6

5

37

123

SOCS 3

0.1

2.1

5.2

14.1

511.05

STAT1

4.25

1.1

2.45

1.05

2

IDO1*

0.05

1.335

5.85

7.125

36.85

FOXO1*

3.15

1.05

4.35

1.05

0.55

BCL2

1

1.75

0.6

2.1

1.05

BCL2l1

8.15

2.2

1.15

4

2.15

TNFRSF10B

8.1

1.05

1.05

4.2

2.1

BIRC5*

3.55

2.35

15.2

4

4.2

ADORA2B

3.75

0.5

22.25

1

0.55

TNFα

0.095

4

0.3

16

16

  1. Monocytes were isolated using human monocyte enrichment cocktail (RosetteSep), and were cultured in presence of growth factors (GM-CSF and IL-4) for 6 days to generated MoDCs, and 200 K-400 K monocytes/well distributed in a six-well plate, and infected immediately with wild type, minor (mfa-1) fimbriae, major (MFI) fimbriae and double (MFB) isogenic mutant(s) of P. gingivalis at 1 MOI. MoDCs and PDDCs were confirmed for their immature DC phenotype (CD14lowCD83−CD1c+DC-SIGN+) on day 6 and 6 hrs post-infection respectively. The gene expression in MoDCs and PDDCs relative to monocytes was performed by using a custom-designed PCR array plates procured from Life technologies. All markers were designed in triplicates on array plates. The fold change (in gene expression) value greater than one indicates a positive value or an up-regulation of gene expression, and less than one indicates a negative value, or down-regulation of gene expression. Asterisks denote significance difference in genes in MoDCs and DPG differentiated DCs.
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