Figure 4: c-Myb expression was decreased in cochlear HCs and HEI-OC1 cells after neomycin exposure.
From: c-Myb knockdown increases the neomycin-induced damage to hair-cell-like HEI-OC1 cells in vitro
(a) Immunofluorescence staining showed that c-Myb expression in cochlear HCs was decreased during neomycin treatment. Mice were given daily subcutaneous injections of neomycin (200 mg/kg) from P7 to P14. At P17, cochlear sensory epithelium samples were dissected out. Mice in the control group were given sterile saline. Scale bars = 30 μm. (b and c) The western blot analysis confirmed that the protein level of c-Myb in cochlear HCs was decreased during neomycin treatment. (d) Immunofluorescence staining in HEI-OC1 cells showed that the expression of c-Myb was decreased after neomycin treatment. The control group was HEI-OC1 cells without any treatment. Scale bars = 5 μm. (e) The mRNA level of c-Myb in HEI-OC1 cells was reduced with the increasing neomycin exposure time. GAPDH was used as a housekeeping gene for control purposes. (f) The protein level of c-Myb in HEI-OC1 cells decreased in a time-dependent manner. β-actin served as the loading control in each lane. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3.
