Figure 6: c-Myb knockdown increased HEI-OC1 cell apoptosis after neomycin treatment.
From: c-Myb knockdown increases the neomycin-induced damage to hair-cell-like HEI-OC1 cells in vitro
All control groups were HEI-OC1 cells without any treatment. (a–c) Flow cytometry analysis in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. Annexin V labels the apoptotic cells and propidium iodide (PI) labels all dying HEI-OC1 cells. (d) Representative images of TUNEL staining in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. (e) Representative images of active caspase-3 staining in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. (f) Quantification of TUNEL-positive cells in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. (g) Quantification of active caspase-3–positive cells in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. (h) qRT-PCR was used to measure the mRNA levels of Apaf-1, Bax, caspase-3, and caspase-9 in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. (i) The western blot was used to measure the protein levels of cleaved caspase-3 and cleaved caspase-9 in the control, shRNA-Control, and shRNA-Myb groups after neomycin exposure. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3. Scale bars = 30 μm.
