Figure 8: HIF-1α knockdown during reperfusion in proximal tubule cells HK2 promotes cell cycle progression. | Scientific Reports

Figure 8: HIF-1α knockdown during reperfusion in proximal tubule cells HK2 promotes cell cycle progression.

From: HIF-1α induction during reperfusion avoids maladaptive repair after renal ischemia/reperfusion involving miR127-3p

Figure 8

HIF-1α was interfered by specific siRNA in HK-2 cells and 48 h later, cells were submitted to H/R protocol. Distribution of HK2 cells in the cell cycle phases was determined by means of propidium iodide staining and flow cytometer analysis (n = 3 in each experimental group). Quantification of cell percentage in each cell cycle phase was performed by Modfit 2.0 software (Beckton Dickinson). The percentage of cells in S replicative phase is significantly increased in HIF-1α knockdown cells compare to scrambled group, at 24 h of reoxygenation. Asterisks indicate statistical significance (*P < 0.05, +/− s.d.). Cell culture, in vitro HIF-1α inhibition and cell cycle distribution assay were performed during 2011.

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