Table 3 Primers sequences used in qRT-PCR for genes.
GENE | PRIMER | SECUENCE 5′–3′ |
|---|---|---|
28S | Forward | CAGTACGAATACAGACCG |
Reverse | GGCAACAACACATCATCAG | |
HIF-1 α | Forward | TCCTATGTGCTGGCTTTGG |
Reverse | AGTGTACCCTAACTAGCCG | |
TGF-β | Forward | CAACAATTCCTGGCGTTACC |
Reverse | TATTCCGTCTCCTTGGTTCAG | |
α-SMA | Forward | ACCCAGATTATGTTTGAGACC |
Reverse | AGAGTCCAGCACAATACCAG | |
IL-1β | Forward | CACCTCTCAAGCAGAGCACAG |
Reverse | GGGTTCCATGGTGAAGTCAAC | |
TNFα | Forward | CCAGGAGAAAGTCAGCCTCCT |
Reverse | TCATACCAGGGCTTGAGCTCA | |
MCP-1 | Forward | CTGCTACTCATTCACTGGC |
Reverse | CTTCTGGACCCATTCCTTATTG | |
Collagen | Forward | GTGGAAACCTGATGTATGCT |
Reverse | TGGTGATACATATTCTTCTGGG | |
BCL6 | Forward | CCGCTACAAAGGCAACCTC |
Reverse | AGCGGTAGGGTTTTTCACCT | |
E-Cadherin (human) | Forward | ACACCAACGATAATCCTCCGA |
Reverse | CATCAGCATCAGTCACTTTCAG | |
α-SMA (human) | Forward | GAGAAGAGTTACGAGTTGCC |
Reverse | ATGATGCTGTTGTAGGTGGT | |
Collagen I (human) | Forward | CTGGAAAGAATGGAGATGATGG |
Reverse | CCAAACCACTGAAACCTCTG |
