Figure 1: Effects of MDPD on NAE 12:0-induced seedling growth inhibition. | Scientific Reports

Figure 1: Effects of MDPD on NAE 12:0-induced seedling growth inhibition.

From: A chemical genetic screen uncovers a small molecule enhancer of the N-acylethanolamine degrading enzyme, fatty acid amide hydrolase, in Arabidopsis

Figure 1

(a) Structure of 6-(2-methoxyphenyl)-1,3-dimethyl-5-phenyl-1H-pyrrolo[3,4-d]pyrimidine-2,4(3 H,6 H)-dione (MDPD). (b) Wild type and AtFAAH overexpressors (AtFAAH OE) seedlings grown for 10 days with or without NAE 12:0 and MDPD. Note that wild-type seedlings on NAE 12:0 and MDPD mirror the growth of AtFAAH OE on NAE 12:0 alone. (c) NAE 12:0 inhibits root hair formation in wild-type seedlings but this is reversed in seedlings treated with both NAE 12:0 and MDPD. (d) Root length of wild-type seedlings on 30 μM NAE 12:0 alone or 30 μM NAE 12:0 plus 30 μM, 50 μM or 100 μM MDPD. Note that increasing MDPD concentrations progressively attenuates NAE 12:0-induced inhibition of root elongation. (e) Root hair length in MDPD and NAE 12:0 is significantly longer than root hair length in solvent control (DMSO) and reversed NAE 12:0-induced root hair growth inhibition. (f,g) Cotyledon area of seedlings on media supplemented with 30 μM or 40 μM NAE 12:0 and different concentrations of MDPD. Adding MDPD to the growth medium lessens the growth inhibitory effects of NAE 12:0 on cotyledon size. Error bars represent the standard error of the means (n ≥ 16 for cotyledon area; n ≥ 120 for primary root and root hair length. Means with different letters are significantly different (P < 0.005; Tukey’s test).

Back to article page